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Download PDF (1045K) All cells described above were incubated at 37°C in a humidified atmosphere of 5% CO2 in air. A Luna C18-column (150×4.6 mm, 5 µ particle size) (Phenomenex, Torrance, CA, U.S.A.) was used to separate retinoids. NOZ C-1 cells (1×106 cells, 0.2 mL) suspended in sterile phosphate-buffered saline (1.5 mM KH2PO4, 8.1 mM Na2HPO4, 136.9 mM NaCl, pH 7.2) (PBS).

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Download PDF (1045K) All cells described above were incubated at 37°C in a humidified atmosphere of 5% CO2 in air. A Luna C18-column (150×4.6 mm, 5 µ particle size) (Phenomenex, Torrance, CA, U.S.A.) was used to separate retinoids. NOZ C-1 cells (1×106 cells, 0.2 mL) suspended in sterile phosphate-buffered saline (1.5 mM KH2PO4, 8.1 mM Na2HPO4, 136.9 mM NaCl, pH 7.2) (PBS).

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